Fishes, Free Full-Text

Por um escritor misterioso

Descrição

Non-specific cytotoxic cell l (NCC) is a kind of important lymphocyte participating in the non-specific immune response in teleost. Non-specific cytotoxic cell receptor protein 1 (NCCRP-1) is a receptor molecule on the surface of NCC and plays an important role in mediating the activity of NCC. However, there are few reports on which signal molecule could transmit signals through NCCRP-1. In this study, yeast two-hybrid library of tilapia liver and head kidney was constructed, and a transferrin from Oreochromis niloticus (On-TF) with interaction protein sequence was obtained by screening the library with bait vector NCCRP-1 of Oreochromis niloticus (On-NCCRP-1). Then, the open reading frame (ORF) of On-TF was cloned, which had 2088 bp in length, encoding polypeptides of 695 amino acids. The deduced amino acid sequence was highly homologous to teleost and similar to mammalian TF, containing two TR_FER domains (25-343th aa and 344-686th aa) for binding iron ions. Furthermore, a point-to-point yeast two-hybrid method was used to further verify whether On-NCCRP-1 could bind to On-TF. The recombinant On-TF (rOn-TF) protein was purified by prokaryotic expression system. In vitro experiments showed that rOn-TF could up-regulate the expression of killing effector molecule of NCC by On-NCCRP-1, and rOn-TF-activated NCCs showed a significantly improved ability to kill FHM cells, indicating that rOn-TF could regulate the NCC signaling pathway through NCC receptor molecule On-NCCRP-1. The results provide a more theoretical basis for understanding the regulation mechanism of NCC activity.

FUN, BRIGHT PATTERNS, AND COLORS: CGK sheets are perfect for boys and girls, and toddlers with fun patterns making bedtime more exciting! Your son or

Kids Fish Blue Full 4 Piece Sheet Set – Boys, Girls, Teens, Toddler – Easy Fit Deep Pockets – Breathable, Hotel Quality Bedding Sheets - Machine

Instant Download , DIGITAL FILE- NO PHYSICAL PRODUCT WILL BE SENT, This is Digital artwork ready for immediate download and ready to be use on such

, Welcome to Pixeo - Your Favorite Shop:, ⚡️ INSTANT DOWNLOAD ⚡️, This is a digital product no

Grouper Fishing Svg, Fishing Svg, Grouper Fish Svg, Grouper Clipart, Grouper Vector, Grouper Cricut, Grouper Cut file, Fish Svg, Grouper Svg

Fish Mongers - King of all fishes - Seer Fish/Vanjaram/Surmai @₹599/Kg! Almost no smell, only one center bone, and immense health benefits - contains a high quantity of protein, calcium, phosphorus, and